Posts tagged experiments
CU study hints conditions on Mars may support energy for life forms
May 30th
A chemical reaction between iron-containing minerals and water may produce enough hydrogen “food” to sustain microbial communities living in pores and cracks within the enormous volume of rock below the ocean floor and parts of the continents, according to a new study led by the University of Colorado Boulder.
The findings, published in the journal Nature Geoscience, also hint at the possibility that hydrogen-dependent life could have existed where iron-rich igneous rocks on Mars were once in contact with water.
Scientists have thoroughly investigated how rock-water reactions can produce hydrogen in places where the temperatures are far too hot for living things to survive, such as in the rocks that underlie hydrothermal vent systems on the floor of the Atlantic Ocean. The hydrogen gases produced in those rocks do eventually feed microbial life, but the communities are located only in small, cooler oases where the vent fluids mix with seawater.
The new study, led by CU-Boulder Research Associate Lisa Mayhew, set out to investigate whether hydrogen-producing reactions also could take place in the much more abundant rocks that are infiltrated with water at temperatures cool enough for life to survive.
“Water-rock reactions that produce hydrogen gas are thought to have been one of the earliest sources of energy for life on Earth,” said Mayhew, who worked on the study as a doctoral student in CU-Boulder Associate Professor Alexis Templeton’s lab in the Department of Geological Sciences.
“However, we know very little about the possibility that hydrogen will be produced from these reactions when the temperatures are low enough that life can survive. If these reactions could make enough hydrogen at these low temperatures, then microorganisms might be able to live in the rocks where this reaction occurs, which could potentially be a huge subsurface microbial habitat for hydrogen-utilizing life.”
When igneous rocks, which form when magma slowly cools deep within the Earth, are infiltrated by ocean water, some of the minerals release unstable atoms of iron into the water. At high temperatures — warmer than 392 degrees Fahrenheit — scientists know that the unstable atoms, known as reduced iron, can rapidly split water molecules and produce hydrogen gas, as well as new minerals containing iron in the more stable, oxidized form.
Mayhew and her co-authors, including Templeton, submerged rocks in water in the absence of oxygen to determine if a similar reaction would take place at much lower temperatures, between 122 and 212 degrees Fahrenheit. The researchers found that the rocks did create hydrogen — potentially enough hydrogen to support life.
To understand in more detail the chemical reactions that produced the hydrogen in the lab experiments, the researchers used “synchrotron radiation” — which is created by electrons orbiting in a manmade storage ring — to determine the type and location of iron in the rocks on a microscale.
The researchers expected to find that the reduced iron in minerals like olivine had converted to the more stable oxidized state, just as occurs at higher temperatures. But when they conducted their analyses at the Stanford Synchrotron Radiation Lightsource at Stanford University, they were surprised to find newly formed oxidized iron on “spinel” minerals found in the rocks. Spinels are minerals with a cubic structure that are highly conductive.
Finding oxidized iron on the spinels led the team to hypothesize that, at low temperatures, the conductive spinels were helping facilitate the exchange of electrons between reduced iron and water, a process that is necessary for the iron to split the water molecules and create the hydrogen gas.
“After observing the formation of oxidized iron on spinels, we realized there was a strong correlation between the amount of hydrogen produced and the volume percent of spinel phases in the reaction materials,” Mayhew said. “Generally, the more spinels, the more hydrogen.”
Not only is there a potentially large volume of rock on Earth that may undergo these low temperature reactions, but the same types of rocks also are prevalent on Mars, Mayhew said. Minerals that form as a result of the water-rock reactions on Earth have been detected on Mars as well, which means that the process described in the new study may have implications for potential Martian microbial habitats.
Mayhew and Templeton are already building on this study with their co-authors, including Thomas McCollom at CU-Boulder’s Laboratory for Atmospheric and Space Physics, to see if the hydrogen-producing reactions can actually sustain microbes in the lab.
This study was funded by the David and Lucille Packard Foundation and with a U.S. Department of Energy Early Career grant to Templeton.
-CU-
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CU Boulder study: More power leads to more dehumanization (No sh*t, Sherlock)
Mar 6th
The study, to be published in the May issue of the Journal of Experimental Social Psychology, found that participants given more powerful roles in two experiments attributed fewer uniquely human traits — characteristics that distinguish people from other animals — to their peers who were given less powerful roles.
“I think a lot of us have the intuition that some powerful people can be pretty dehumanizing,” said Jason Gwinn, a doctoral student in the Department of Psychology and Neuroscience and lead author of the study. “But our goal was to test if power, when randomly assigned to ordinary students, would have that effect. That would say something about power itself rather than about the sort of people who have the drive to take power.”
The researchers enlisted about 300 CU-Boulder students taking an introductory psychology course to participate in two experiments. In the first experiment, students were assigned to be either a manager or an assistant for a mock hiring task. The assistants were asked to review resumes for an open job and then list the strengths and weaknesses of each applicant. The managers then reviewed the list made by their assistants and made a final decision about whom to hire.
In the second experiment, participants were asked to play a game and were assigned to be either an allocator or a recipient. For the game, one allocator and one recipient were tasked with splitting a pot of money. The allocator, the higher-power role, made the first offer, suggesting how the money be split. If the recipient, the lower-power role, accepted the offer, both people received their share of the money. If the recipient declined the offer, neither person received any of the money.
At the end of each experiment, the participants were asked to rate each other on 40 traits. The result was that students in higher-power roles assigned fewer uniquely human traits to the students in lower-power roles than vice versa. Examples of traits considered to be more uniquely human, as defined and tested in a 2007 Australian study, include being ambitious, imaginative, frivolous and insecure. Examples of traits that are less uniquely human — those that could be used to describe a pet as well as a friend, for example — include being passive, timid, friendly and shy.
The question of whether power leads to dehumanization has part of its roots in the renowned Stanford Prison Experiment conducted in 1971. Twenty-four male students were randomly assigned to play the role of either inmate or guard in a mock prison in the basement of the Stanford psychology building. During the study, the guards were psychologically abusive to the prisoners, many of whom passively accepted the abuse, despite the fact that the participants knew that they were all students at the same elite university.
Though the guards were described as dehumanizing the prisoners, the term “dehumanization” was well defined at the time and the experiment was not designed to allow the researchers to confidently state that it was the increase in power that lead to the dehumanization. By contrast, Gwinn’s study, now available online, was designed specifically to test the relationship between power and dehumanization.
Gwinn cautions that the researchers cannot yet say whose perspective is being changed by the power differential imposed on participants in the CU study. It’s possible that being in a position of less power makes a person see those in power as more human rather than the other way around, or that both people are affected.
“We haven’t pinned down why this happens,” Gwinn said. “We don’t know whose perception is being affected.”
Charles Judd and Bernadette Park, both professors in CU-Boulder’s Department of Psychology and Neuroscience, co-authored the study.
CU scientists seek solution to the mystery of ever-increasing frog deformities
Feb 14th
CU-Boulder amphibian study shows how
biodiversity can protect against disease
The richer the assortment of amphibian species living in a pond, the more protection that community of frogs, toads and salamanders has against a parasitic infection that can cause severe deformities, including the growth of extra legs, according to a new study by the University of Colorado Boulder.
The findings, published Feb. 14 in the journal Nature, support the idea that greater biodiversity in larger-scale ecosystems, such as forests or grasslands, may also provide greater protection against diseases, including those that attack humans. For example, a larger number of mammal species in an area may curb cases of Lyme disease, while a larger number of bird species may slow the spread of West Nile virus.
“How biodiversity affects the risk of infectious diseases, including those of humans and wildlife, has become an increasingly important question,” said Pieter Johnson, an assistant professor in the Department of Ecology and Evolutionary Biology and lead author of the study. “But as it turns out, solidly testing these linkages with realistic experiments has proven very challenging in most systems.”
Researchers have struggled to design comprehensive studies that could illuminate the possible connection between disease transmission and the number of species living in complex ecosystems. Part of the problem is simply the enormous number of organisms that may need to be sampled and the vast areas over which those organisms may roam.
The new CU-Boulder study overcomes that problem by studying smaller, easier-to-sample ecosystems. Johnson and his team visited hundreds of ponds in California, recording the types of amphibians living there as well as the number of snails infected by the pathogen Ribeiroia ondatrae. Snails are an intermediate host used by the parasite during part of its life cycle.
“One of the great challenges in studying the diversity-disease link has been collecting data from enough replicate systems to differentiate the influence of diversity from background ‘noise,’ ” Johnson said. “By collecting data from hundreds of ponds and thousands of amphibian hosts, our group was able to provide a rigorous test of this hypothesis, which has relevance to a wide range of disease systems.”
Johnson’s team buttressed its field observations both with laboratory tests designed to measure how prone to infection each amphibian species is and by creating pond replicas outside using large plastic tubs stocked with tadpoles that were exposed to a known number of parasites. All of the experiments told the same story, Johnson said. Greater biodiversity reduced the number of successful amphibian infections and the number of deformed frogs.
A normal and a deformed bullfrog
In all, the CU-Boulder researchers spent three years sampling 345 wetlands and recording malformations — which include missing, misshapen or extra sets of hind legs — caused by parasitic infections in 24,215 amphibians. They also cataloged 17,516 snails. The results showed that ponds with half a dozen amphibian species had a 78 percent reduction in parasite transmission compared to ponds with just one amphibian species. The research team also set up experiments in the lab and outdoors using 40 artificial ponds, each stocked with 60 amphibians and 5,000 parasites.
The reason for the decline in parasitic infections as biodiversity increases is likely related to the fact that ponds add amphibian species in a predictable pattern, with the first species to appear being the most prone to infection and the later species to appear being the least prone. For example, the research team found that in a pond with just one type of amphibian, that amphibian was almost always the Pacific chorus frog, a creature that is able to rapidly reproduce and quickly colonize wetland habitats, but which is also especially vulnerable to infection and parasite-induced deformities.
On the other hand, the California tiger salamander was typically one of the last species to be added to a pond community and also one of the most resistant to parasitic infection. Therefore, in a pond with greater biodiversity, parasites have a higher chance of encountering an amphibian that is resistant to infection, lowering the overall success rate of transmission between infected snails and amphibians.
This same pattern — of less diverse communities being made up of species that are more susceptible to disease infection — may well play out in more complex ecosystems as well, Johnson said. That’s because species that disperse quickly across ecosystems appear to trade off the ability to quickly reproduce with the ability to develop disease resistance.
“This research reaches the surprising conclusion that the entire set of species in a community affects the susceptibility to disease,” said Doug Levey, program director in the National Science Foundation’s Division of Environmental Biology, which helped fund the research. “Biodiversity matters.”
The sheer magnitude of the recent study also reinforces the connection between deformed frogs and parasitic infection, Johnson said. Beginning in the mid-1990s reports of frogs with extra, missing or misshapen legs skyrocketed, attracting widespread attention in the media and motivating scientists to try to figure out the cause. Johnson was among the researchers who found evidence of a link between infection with Ribeiroia and frog deformities, though the apparent rise in reports of deformations, and its underlying cause, remains controversial.
While the new study has implications beyond parasitic infections in amphibians, it does not mean that an increase in biodiversity always results in a decrease in disease, Johnson cautioned. Other factors also affect rates of disease transmission. For example, a large number of mosquitoes hatching in a particular year will increase the risk of contracting West Nile virus, even if there has been an increase in the biodiversity of the bird population. Birds act as “reservoir hosts” for West Nile virus, harboring the pathogen indefinitely with no ill effects and passing the pathogen onto mosquitoes.
“Our results indicate that higher diversity reduces the success of pathogens in moving between hosts,” Johnson said. “Nonetheless, if infection pressure is high, for instance in a year with high abundance of vectors, there will still be a significant risk of disease; biodiversity will simply function to dampen transmission success.”
CU-Boulder graduate students Dan Preston and Katie Richgels co-authored the study along with Jason Hoverman, a former postdoctoral researcher in Johnson’s lab who is now an assistant professor at Purdue. The research was funded by NSF, the National Geographic Society and the David and Lucile Packard Foundation.
To view photos and a video about the research, visit http://freshwatersillustrated.org/link/AmphibianDeformities.
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