Posts tagged increase
CU scientists seek solution to the mystery of ever-increasing frog deformities
Feb 14th
CU-Boulder amphibian study shows how
biodiversity can protect against disease
The richer the assortment of amphibian species living in a pond, the more protection that community of frogs, toads and salamanders has against a parasitic infection that can cause severe deformities, including the growth of extra legs, according to a new study by the University of Colorado Boulder.
The findings, published Feb. 14 in the journal Nature, support the idea that greater biodiversity in larger-scale ecosystems, such as forests or grasslands, may also provide greater protection against diseases, including those that attack humans. For example, a larger number of mammal species in an area may curb cases of Lyme disease, while a larger number of bird species may slow the spread of West Nile virus.
“How biodiversity affects the risk of infectious diseases, including those of humans and wildlife, has become an increasingly important question,” said Pieter Johnson, an assistant professor in the Department of Ecology and Evolutionary Biology and lead author of the study. “But as it turns out, solidly testing these linkages with realistic experiments has proven very challenging in most systems.”
Researchers have struggled to design comprehensive studies that could illuminate the possible connection between disease transmission and the number of species living in complex ecosystems. Part of the problem is simply the enormous number of organisms that may need to be sampled and the vast areas over which those organisms may roam.
The new CU-Boulder study overcomes that problem by studying smaller, easier-to-sample ecosystems. Johnson and his team visited hundreds of ponds in California, recording the types of amphibians living there as well as the number of snails infected by the pathogen Ribeiroia ondatrae. Snails are an intermediate host used by the parasite during part of its life cycle.
“One of the great challenges in studying the diversity-disease link has been collecting data from enough replicate systems to differentiate the influence of diversity from background ‘noise,’ ” Johnson said. “By collecting data from hundreds of ponds and thousands of amphibian hosts, our group was able to provide a rigorous test of this hypothesis, which has relevance to a wide range of disease systems.”
Johnson’s team buttressed its field observations both with laboratory tests designed to measure how prone to infection each amphibian species is and by creating pond replicas outside using large plastic tubs stocked with tadpoles that were exposed to a known number of parasites. All of the experiments told the same story, Johnson said. Greater biodiversity reduced the number of successful amphibian infections and the number of deformed frogs.
A normal and a deformed bullfrog
In all, the CU-Boulder researchers spent three years sampling 345 wetlands and recording malformations — which include missing, misshapen or extra sets of hind legs — caused by parasitic infections in 24,215 amphibians. They also cataloged 17,516 snails. The results showed that ponds with half a dozen amphibian species had a 78 percent reduction in parasite transmission compared to ponds with just one amphibian species. The research team also set up experiments in the lab and outdoors using 40 artificial ponds, each stocked with 60 amphibians and 5,000 parasites.
The reason for the decline in parasitic infections as biodiversity increases is likely related to the fact that ponds add amphibian species in a predictable pattern, with the first species to appear being the most prone to infection and the later species to appear being the least prone. For example, the research team found that in a pond with just one type of amphibian, that amphibian was almost always the Pacific chorus frog, a creature that is able to rapidly reproduce and quickly colonize wetland habitats, but which is also especially vulnerable to infection and parasite-induced deformities.
On the other hand, the California tiger salamander was typically one of the last species to be added to a pond community and also one of the most resistant to parasitic infection. Therefore, in a pond with greater biodiversity, parasites have a higher chance of encountering an amphibian that is resistant to infection, lowering the overall success rate of transmission between infected snails and amphibians.
This same pattern — of less diverse communities being made up of species that are more susceptible to disease infection — may well play out in more complex ecosystems as well, Johnson said. That’s because species that disperse quickly across ecosystems appear to trade off the ability to quickly reproduce with the ability to develop disease resistance.
“This research reaches the surprising conclusion that the entire set of species in a community affects the susceptibility to disease,” said Doug Levey, program director in the National Science Foundation’s Division of Environmental Biology, which helped fund the research. “Biodiversity matters.”
The sheer magnitude of the recent study also reinforces the connection between deformed frogs and parasitic infection, Johnson said. Beginning in the mid-1990s reports of frogs with extra, missing or misshapen legs skyrocketed, attracting widespread attention in the media and motivating scientists to try to figure out the cause. Johnson was among the researchers who found evidence of a link between infection with Ribeiroia and frog deformities, though the apparent rise in reports of deformations, and its underlying cause, remains controversial.
While the new study has implications beyond parasitic infections in amphibians, it does not mean that an increase in biodiversity always results in a decrease in disease, Johnson cautioned. Other factors also affect rates of disease transmission. For example, a large number of mosquitoes hatching in a particular year will increase the risk of contracting West Nile virus, even if there has been an increase in the biodiversity of the bird population. Birds act as “reservoir hosts” for West Nile virus, harboring the pathogen indefinitely with no ill effects and passing the pathogen onto mosquitoes.
“Our results indicate that higher diversity reduces the success of pathogens in moving between hosts,” Johnson said. “Nonetheless, if infection pressure is high, for instance in a year with high abundance of vectors, there will still be a significant risk of disease; biodiversity will simply function to dampen transmission success.”
CU-Boulder graduate students Dan Preston and Katie Richgels co-authored the study along with Jason Hoverman, a former postdoctoral researcher in Johnson’s lab who is now an assistant professor at Purdue. The research was funded by NSF, the National Geographic Society and the David and Lucile Packard Foundation.
To view photos and a video about the research, visit http://freshwatersillustrated.org/link/AmphibianDeformities.
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CU study: Southwest regional warming likely cause of pinyon pine cone decline
Feb 12th
Creeping climate change in the Southwest appears to be having a negative effect on pinyon pine reproduction, a finding with implications for wildlife species sharing the same woodland ecosystems, says a University of Colorado Boulder-led study.
The new study showed that pinyon pine seed cone production declined by an average of about 40 percent at nine study sites in New Mexico and northwestern Oklahoma over the past four decades, said CU-Boulder doctoral student Miranda Redmond, who led the study. The biggest declines in pinyon pine seed cone reproduction were at the higher elevation research sites experiencing more dramatic warming relative to lower elevations, said Redmond of CU’s ecology and evolutionary biology department.
“We are finding significant declines in pinyon pine cone production at many of our study sites,” said Redmond. “The biggest declines in cone production we measured were in areas with greater increases in temperatures over the past several decades during the March to October growing season.”
The cones in which the pinyon seeds are produced are initiated two years prior to seed maturity, and research suggests the environmental stimulus for cone initiation is unseasonably low temperatures during the late summer, said Redmond. Between 1969 and 2009, unseasonably low temperatures in late summer decreased in the study areas, likely inhibiting cone initiation and development.
The study is one of the first to examine the impact of climate change on tree species like pinyon pines that, instead of reproducing annually, shed vast quantities of cones every few years during synchronous, episodic occurrences known as “masting” events. Redmond said such masting in the pinyon pine appears to occur every three to seven years, resulting in massive “bumper crops” of cones covering the ground.
In the new Ecosphere study, the researchers compared two 10-year sequences of time. In addition to showing that total pinyon pine cone production during the 2003-2012 decade had declined from the 1969-1978 decade in the study areas, the team found the production of cones during masting events also declined during that period.
Some scientists believe masting events evolved to produce a big surplus of nut-carrying cones — far too many for wildlife species to consume in a season — making it more likely the nuts eventually will sprout into pinyon pine seedlings, she said. Others have suggested masting events occur during favorable climate conditions and/or to increase pollination efficiency. “Right now we really don’t know what drives them,” Redmond said.
“Across a range of forested ecosystems we are observing widespread mortality events due to stressors such as changing climate, drought, insects and fire,” said CU’s Barger. “This study provides evidence that increasing air temperatures may be influencing the ability of a common and iconic western U.S. tree, pinyon pine, to reproduce. We would predict that declines in pinyon pine cone production may impact the long-term viability of these tree populations.”
Wildlife biologists say pinyon-juniper woodlands are popular with scores of bird and mammal species ranging from black-chinned hummingbirds to black bears. A 2007 study by researchers at the University of Northern Arizona estimated that 150 Clark’s Nutcrackers cached roughly 5 million pinyon pine nuts in a single season, benefiting not only the birds themselves but also the pines whose nuts were distributed more widely for possible germination.
For the new study, Redmond revisited nine pinyon pine study sites scattered throughout New Mexico and Oklahoma that had been studied previously in 1978 by Forcella. Both Forcella and Redmond were able to document pinyon pine masting years by counting small, concave blemishes known as “abscission scars” on individual tree branches that appeared after the cones have been dropped, she said.
Since each year in the life of a pinyon pine tree is marked by a “whorl” — a single circle of branches extending around a tree trunk — the researchers were able to bracket pinyon pine reproductive activity in the nine study areas for the 1969-1978 decade and 2003-2012 decade, which were then compared.
Pinyon pines take three growing seasons, or about 26 months, to produce mature cones from the time of cone initiation. Low elevation conifers including pinyon pines grow in water-limited environments and have been shown to have higher cone output during cool and/or wet summers, said Redmond. In addition to the climate-warming trend under way in the Southwest, the 2002-03 drought caused significant mortality in pinyon pine forests, Redmond said.
“Miranda’s ideas and accompanying results will be of value to ecologists and land managers in the deserts of the Southwest and beyond,” said Forcella, now a research agronomist in the USDA’s Agricultural Research Service. “The work is evidence that the University of Colorado continues to cultivate a cadre of high-caliber graduate students for which it rightfully can take tremendous pride.”
Pinyon nuts, the Southwest’s only commercial source of edible pine seeds today, were dietary staples of indigenous Americans going back millennia.
For more information on CU-Boulder’s ecology and evolutionary biology department visit http://ebio.colorado.edu.
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CU researchers: Beetle killed trees are better than logging for watersheds
Jan 14th
CU-led study shows pine beetle outbreak
buffers watersheds from nitrate pollution
buffers watersheds from nitrate pollution
A research team involving several scientists from the University of Colorado Boulder has found an unexpected silver lining in the devastating pine beetle outbreaks ravaging the West: Such events do not harm water quality in adjacent streams as scientists had previously believed.
According to CU-Boulder team member Professor William Lewis, the new study shows that smaller trees and other vegetation that survive pine beetle invasions along waterways increase their uptake of nitrate, a common disturbance-related pollutant. While logging or damaging storms can drive stream nitrate concentrations up by 400 percent for multiple years, the team found no significant increase in the nitrate concentrations following extensive pine beetle tree mortality in a number of Colorado study areas.
Beetle-killed forests do not release nitrogen into the water, according to CU researchers
“We found that the beetles do not disturb watersheds in the same way as logging and severe storms,” said Lewis, interim director of CU’s Cooperative Institute for Research in Environmental Sciences. “They leave behind smaller trees and other understory vegetation, which compensate for the loss of larger pine trees by taking up additional nitrate from the system. Beetle-kill conditions are a good benchmark for the protection of sub-canopy vegetation to preserve water quality during forest management activities.”
A paper on the subject was published in the Jan. 14 issue of the Proceedings of the National Academy of Sciences.
“The U.S. Forest Service and other agencies have established harvesting practices that greatly mitigate damage to forests caused by logging, and they deserve credit for that,” said Lewis. “But this study shows just how important the survival of smaller trees and understory vegetation can be to stream water quality.”
In waterways adjacent to healthy pine forests, concentrations of nitrate is generally far lower than in rivers on the plains in the West like the South Platte, said Lewis. Nitrate pollution is caused by agricultural runoff from populated areas and by permitted discharges of treated effluent from water treatment facilities.
“In Colorado, many watersheds have lost 80 to 90 percent of their tree canopy as a result of the beetle epidemic,” said Lewis, also a faculty member in CU-Boulder’s ecology and evolutionary biology department. “We began to wonder whether the loss of the trees was reducing water quality in the streams. We knew that forestry and water managers were expecting big changes in water quality as a result of the pine beetle outbreak, so we decided to pool our university and federal agency resources in order to come up with an answer.”
Study co-author and CU-Boulder Research Associate James McCutchan of CIRES said the new results should help forest managers develop more effective ways to harvest timber while having the smallest effect possible on downstream ecosystems. “This study shows that at least in some areas, it is possible to remove a large part of the tree biomass from a watershed with a very minimal effect on the stream ecosystem,” he said.
Understory vegetation left intact after beetle outbreaks gains an ecological advantage in terms of survival and growth, since small trees no longer have to compete with large trees and have more access to light, water and nutrients, said McCutchan. Research by study co-author and former CU undergraduate Rachel Ertz showed concentrations of nitrate in the needles of small pines that survived beetle infestations were higher than those in healthy trees outside beetle-killed areas, another indication of how understory vegetation compensates for environmental conditions in beetle kill areas.
The researchers used computer modeling to show that in western forests, such a “compensatory response” provides potent water quality protection against the adverse effects of nitrates only if roughly half of the vegetation survives “overstory” mortality from beetle kill events, which is what occurs normally in such areas, said Lewis.
Other study co-authors included Leigh Cooper, Thomas Detmer and Thomas Veblen from CU-Boulder, John Stednick from Colorado State University, Charles Rhoades from the U.S. Forest Service, Jennifer Briggs and David Clow from the U.S. Geological Survey and Gene Likens of the Cary Institute of Ecosystem Studies in Millbrook, N.Y.
The severe pine beetle epidemic in Colorado and Wyoming forests is part of an unprecedented beetle outbreak that ranges from Mexico to Canada. A November 2012 study by CU-Boulder doctoral student Teresa Chapman showed the 2001-02 drought greatly accelerated the development of the mountain pine beetle epidemic.
The researchers measured stream nitrate concentrations at more than 100 sites in western Colorado containing lodgepole pines with a range of beetle-induced tree damage. The study area included measurements from the Fraser Experimental Forest near Granby, Colo., a 23,000-acre study area established by the USFS in 1937.
The new study was funded by the USFS, the USGS, the National Science Foundation, the National Oceanic and Atmospheric Administration and the National Park Service. CIRES is a joint research institute between CU-Boulder and NOAA.
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